Mouse Anti-EBV particles Hybridoma [83B2]
This hybridoma produces mAbs against EBV particles
COA
GeneralInformation
Immunogen
Epstein-barr virus (ebv) particles
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
BALB/c mouse spleen
Clone
83B2
Cell Line Description
Established by fusion of the P3X62AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with Epstein-Barr virus (EBV) particles; cells produce a monoclonal antibody (igg1) that reacts with a viral envelope glycoprotein (350/220 kd) and that neutralizes EBV confirmed as mouse with IEF of AST, MDH, PEP B. .
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV
Morphology
single cells in suspension
Growth Properties
suspension
CultureMethod
Subculturing
Split ratio: 1:3 to1: 5, every 2-3 days; seed out at 0.1-1.0 x 10^6 cells/ml; Harvest: maximal density of about 1.0-1.5 x 10^6 cells/ml.
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium(20%FBS), add 10%(v/v) DMSO
Freezing medium: to complete growth medium(20%FBS), add 10%(v/v) DMSO
Citations
Have you cited CSC-H1427 in a publication? Let us know and earn a reward for your research.
| Product Name | Cat. No. | Applications | Host Species | Datasheet | Price | Add to Basket |
|---|
| Product Name | Cat. No. | Applications | Host Species | Datasheet | Price | Add to Basket |
|---|
Croia, C; Serafini, B; et al. Epstein-Barr virus persistence and infection of autoreactive plasma cells in synovial lymphoid structures in rheumatoid arthritis. ANNALS OF THE RHEUMATIC DISEASES 72:1559-1568(2013).
Casabonne, D; Benavente, Y; et al. Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study. INFECTIOUS AGENTS AND CANCER 10:-(2015).
EBV Related Products
Online Inquiry
Catalog # CSC-H1427
Related Products
By targets
Related Resources
-
Cancer Research
- B&T Cell-Associated Lymphoma
- Bladder Cancer Panel
- Brain Tumor Panel
- Cervical Cancer Panel
- Colorectal and Gastric Cancer Panel
- Esophagus Cancer Panel
- Gastrointestinal Cancer
- Genito-Urinary Cancer
- Germ Cell Tumor
- Granulocyte Products
- Head and Neck Cancer
- Kidney Cancer
- Liver Cancer
- Lung Cancer
- Melanoma
- Molecular Targets of Glycolysis
- Muscle Tumor
- Neuroendocrine Tumors
- Ovarian Cancer
- Pancreatic Cancer
- Papillary Thyroid Cancer
- Pituitary Gland Tumor
- Prostate and Testicular Cancer
- Skin Biopsy
- Skin Cancer
- Vascular Cancer
- Gene Therapy
-
Immunology Topics
- Antibody Structure and Function
- Antibody Validation
- Autoantigen Proteins in T1DM Research
- Autoimmune Diseases
- Bacterial Toxins
- Bordetella Pertussis Toxin
- Campylobacter
- Cardiac Disease
- Childhood Diseases
- Coronavirus
- Dengue Virus
- Drugs of Abuse
- Enteroviruses
- F4/80 Antibody: Mouse Macrophage and Microglial Marker
- Features and Applications of VHH Molecules
- Flavivirus
- Functions and Regulation of Proglucagon Family Peptides
- Granulocytes
- How do Th1 and Th2 Cells Differentiate
- Immunoglobulin: Types, Classes and Subclasses
- Japanese Encephalitis Virus
- Macrophage migration inhibitory factor (MIF)
- Macrophages and Their Markers
- Marine Biotoxins
- Monkeypox Virus
- Phagocytic Cells and The Reticuloendothelial System
- Phagocytosis
- Quantitative Antibody Affinity Measurement
- SARS-CoV-2
- The Application of IHC in Pathological Diagnosis
- Therapeutic Drug Monitoring (TDM)
- ToRCH
- Veterinary Infectious Diseases
- Virus-Like Particles (VLPs)
- West Nile Virus
- Yellow Fever Virus
- Zika Virus
- IVD Raw Materials
-
Signaling Pathway
- Cancer Research
- ALK Pathway
- Angiogenesis Signaling Pathway
- APC/C Signaling Pathway
- CDK Signaling Pathway
- DNA-PK Signaling Pathway
- EGF/EGFR Signaling Pathway
- Hallmarks of Cancer
- KLF4 Signaling Pathway
- MELK Signaling Pathway
- PDK-1 Signaling Pathway
- PLK Signaling Pathway
- ROCK Signaling Pathway
- ROS1 Signaling Pathway
- S6 Kinase Signaling Pathway
- Tumor Immunology Pathway
- Wee1 Signaling Pathway
- Cell Biology
- Apoptosis Execution Pathway
- Autophagy
- Calcium Signaling Pathway
- Cell Senescence Signaling Pathway
- Chk Signaling Pathway
- C-MYC Signaling Pathway
- CXCR Signaling Pathway
- Epigenetics
- Exosomes Pathway
- Extrinsic Apoptosis Pathway
- GPCR Pathway
- Intrinsic Apoptosis Pathway
- LRRK2 Signaling Pathway
- Necroptosis Signaling Pathway
- OCT3/4 Signaling Pathway
- Perforin/Granzyme Apoptosis Pathway
- Proliferation
- Protein Phosphorylation
- Rho Signaling Pathway
- Sox2 Signaling Pathway
- TGF-β/SMAD Signaling Pathway
- Ubiquitin-Proteasome Pathway
- Endocrine System
- Genetics
- Aurora Kinase Signaling Pathway
- Base Excision Repair Pathway
- DNA Methyltransferase Pathway
- Histone Acetyltransferase Pathway
- Histone Deacetylase Pathway
- Histone Demethylation Pathway
- Histone Methyltransferase Signaling Pathway
- Homologous Recombination Pathway
- Mismatch Repair Pathway
- Non-homologous End-joining Pathway
- Sirtuin Signaling Pathway
- Immune Checkpoint
- 4-1BB/4-1BBL Signaling Pathway
- CD27/CD70 Signaling Pathway
- CD40/CD40L Signaling Pathway
- CTLA-4/CD28 Signaling Pathway
- HVEM/BTLA/CD160 Signaling Pathway
- Immune Checkpoint Signaling Pathway
- LAG3/MHC Class II Signaling Pathway
- OX40/OX40L Signaling Pathway
- PD-1/PD-L1 Signaling Pathway
- TIM-3/Galectin-9 Signaling Pathway
- Metabolism
- Adiponectin Signaling Pathway
- AMPK Signaling Pathway
- Apelin Signaling Pathway
- Chemerin Signaling Pathway
- Diabetes Signaling Pathway
- Fatty Acid Oxidation
- Hepatocyte Growth Factor Signaling Pathway
- IGF-1 Signaling Pathway
- IL-6 Signaling Pathway
- Leptin Signaling Pathway
- Lipid Metabolism Signaling Pathway
- Lipocalin Signaling Pathway
- Monocyte Chemotactic Protein-1 Signaling Pathway
- Nerve Growth Factor Signaling Pathway
- Obese Signaling Pathway
- Omentin Signaling Pathway
- Plasminogen Activator Inhibitor-1 Signaling Pathway
- Resistin Signaling Pathway
- Retinol Binding Protein-4 Signaling Pathway
- TNF-α Signaling Pathway
- Vaspin Signaling Pathway
- Warburg Effect Signaling Pathway
- Neuroscience
- NF-κB Small Molecule Guide
- Cancer Research
-
Technical Documents
- Calculation
- Guides
- In Situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH)
- Antibody Specificity Validation
- Chemiluminescence Immunoassay Guide
- Conjugated Secondary Antibodies
- Direct vs Indirect Immunofluorescence: Which is the Better Technique
- ELISA Development Guide
- ELISA Guide
- Flow Cytometry Guide
- How to Choose An Isotype Control Antibody
- Immunoassay
- Immunochromatography Guide
- Immunofluorescence Guide
- Immunohistochemistry Guide
- Immunoprecipitation Guide
- Nucleic Acid Lateral Flow Immunoassay (NALFIA)
- Unconjugated Secondary Antibodies
- What Is A Clone Number
- Protocol
- Cell Experiment Protocol
- In Vitro Differentiation of T Cells Protocol
- In Vitro Polarization of Murine Macrophage Protocol
- Annexin V-FITC Staining Protocol for Apoptosis Detection
- Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry Protocol
- Counting Cells Using a Hemocytometer
- Flow Cytometric Analysis of Microparticles Protocol
- Flow Cytometric FRET Analysis of Protein Interaction Protocol
- Flow Cytometry
- Flow Cytometry Assays in Primary Immunodeficiency Diseases Protocol
- Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia Protocol
- Flow Cytometry Protocol for Tracking Immune Cell
- Flow Cytometry Protocol: Intracellular Staining Protocol
- Flow Cytometry Protocol: Sample Preparation Protocol
- Flow Cytometry-Based Identification of Immature Myeloerythroid Development Protocol
- Flow-Based Combinatorial Antibody Profiling An Integrated Approach to Cell Characterization Protocol
- Fluorescent Protein-Assisted Purification for Gene Expression Profiling Protocol
- Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics Protocol
- Mammalian Cell Tissue Culture
- Mouse Naïve CD4+ T Cells Isolation Protocol
- Multiparameter Cell Cycle Analysis Protocol
- Multiparameter Intracellular Cytokine Staining
- Multiparametric Analysis of Apoptosis by Flow Cytometry Protocol
- Multiparametric Analysis of Plant Protoplasts and Nuclei Protocol
- Noncytotoxic DsRed Derivatives for Whole-Cell Labeling Protocol
- Phospho-Flow Cytometry Protocol for Analysis of Kinase Signaling in Samples
- Quantitative Fluorescence Measurements with Multicolor Flow Cytometry Protocol
- Quantum Dots for Quantitative Flow Cytometry Protocol
- Rare Event Detection and Analysis in Flow Cytometry Protocol
- Standard Practice for Cell Sorting in a BSL-3 Facility Protocol
- Elisa Experiment Protocol
- Affinity Purification of Antibodies Protocol
- Antibody Detection Protocol with Protein-A Colloidal Gold Flow-Through Assay
- Antibody Labeling with HRP
- Bioconjugation of Antibodies to Horseradish Peroxidase (HRP) Protocol
- Competitive ELISA Protocol
- Correlative Microscopy Protocol for Localization of Proteins In Situ
- Direct ELISA Experimental Protocol
- Direct ELISA Protocol
- Double-Sandwich ELISA Protocol
- ELISpot & DC-ELISpot Measure IFNγ-Secreting Cells Protocol
- Gas Plasma Surface Chemistry for Biological Assays Protocol
- Hybridoma Technology Protocol
- Immuno-PCR Assay Protocol
- In Situ Proximity Ligation Assay (PLA) Protocol
- Indirect ELISA Experimental Protocol
- Indirect ELISA Protocol
- Lateral Flow Immunoassay Protocol
- Making Immunofluorescence Signals Stronger Using Tyramide Protocol
- Multiplex ELISA Using Oligonucleotide Tethered Antibodies Protocol
- Multiplex Immunoassay Protocol
- Multiplexed Microsphere Suspension Array-Based Immunoassays Protocol
- Sandwich ELISA Protocol
- The Biochemical Properties of Antibodies and Their Fragments Protocol
- ELISAPot Experiment Protocol
- Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay Protocol
- Counting Bovine IFN-γ-Secreting Cells using ELISPOT Protocols
- Cross-laboratory ELISPOT Protocol Standard
- Detecting Vaccinia Virus Response in Human Blood Cells Using ELISPOT
- Dual- and Triple-Color Fluorospot Protocol
- ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes Protocol
- ELISPOT Protocol for Detection of Cell Secretion Activity
- ELISPOT Protocol for Detection of TNFα Secreted by Microglia
- ELISPOT Protocol's Membranes and Membrane Plates
- ELISPOT Study the Stem Cells on Cytokine Secretion Protocol
- Equine ELISPOT Assay to Study IFNg and IL-4 Protocol
- IL-7 Increases Spot Size and Number by T-SPOT.TB Protocol
- Mathematical Algorithms for Spotting in the ELISPOT Protocol
- Single Cells' Cytokine Secretion Measurement by ELISPOT and ELISA Protocol
- Statistical Analysis of ELISPOT Assays Protocol
- The Impact of Harmonization on ELISPOT Assay Performance Protocol
- Unique-Strengths of ELISPOT for T Cell Diagnostics Protocol
- User-Independent ELISPOT Data Analysis Protocol
- Utilization of Feline ELISPOT for Mapping Vaccine Epitopes Protocol
- IHC & ICC Experiment Protocol
- Antigen Retrieval and Signal Amplification Protocol
- Comet Assay for the Detection of DNA Breaks Protocol
- Culture and Phenotyping Protocol of Glial Cell, Gliospheres, and Neurospheres
- Detection of Circulating and Tissue MDSC by Flow Cytometry Protocol
- Double Labeling Fluorescent Immunocytochemistry Protocol
- Enzymatic Immunohistochemistry Protocol
- ER+ Breast Cancer Mammosphere Formation and Analysis Protocol
- Fluorescent Immunohistochemistry Protocol
- Fluorescent In Situ Hybridization (FISH) Protocol
- Fluorescent-Activated Cell Sorting (Flow Cytometry) Protocol
- IHC Antigen Retrieval Protocol
- Immunohistochemistry in the Central Nervous System Protocol
- In Situ Hybridization Protocol
- Multiplexing and Spectral Microscopy Protocol
- PLA Protocol
- Primary Antibody Selection and Blocking Techniques for IHC Protocol
- Probes for Fluorescent Visualization of Specific Cellular Organelles Protocol
- RNA Isolation and Reverse Transcription Protocol
- Tissue Processing Protocol
- Protein Experiment Protocol
- Analysis of Antibody Clonotype by Affinity Immunoblotting Protocol
- Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates Protocol
- Buffer and Stock Solution for Western Blot
- Centrifuge Blotting Protocol
- Chromatin Immunoprecipitation (ChIP) Protocol
- Co-Immunoprecipitation (Co-IP) Protocol
- Detection and Quantification of Protein–Protein Interactions by Far-Western Blotting Protocol
- Diffusion Blotting Protocol
- Dot-Immunobinding Assay (Dot-Iba) Protocol
- Double-Blotting Protocol
- Grid-Immunoblotting Protocol
- Immunofluorescence Protocol: Cultured Cell Protocol
- Immunofluorescence protocol: Stick Section Protocol
- Immunoprecipitation (IP) Protocol
- Membrane Strip Affinity Purification of Autoantibodies Protocol
- Polyclonal vs. Monoclonal Antibodies Protocol
- Ribonucleoprotein Immunoprecipitation (RIP) Protocol
- Single-Cell Western Blotting Protocol
- Strip Immunoblotting of Multiple Antigenic Peptides Protocol
- The Basis of Western Blot Protocol
- The Dot Blot Protocol
- Western Blot Protocols (part 1) - Sample & Gel Preparation Protocol
- Western Blot Protocols (part 2) - Electrophoresis & Protein Transfer Protocol
- Western Blot Protocols (part 3) - Antibody Incubation & Gel Visualization Protocol
- Western Blotting Using Microfluidics Protocol
- Method for Determination of Protein Concentration by Absorbance at 280nm Protocol
- Determination of Protein Concentration by the Smith Bicinchoninic Acid Method Protocol
- Determination of Protein Concentration by the Bradford Coomassie Blue G-250 Method Protocol
- Determination of Protein Concentration by the Lowry Method Protocol
- BrdU Staining Protocol
- Western Blot Membrane Stripping for Restaining Protocol
- Loading Controls for Western Blotting
- Sample Praparetion Protocol
- Cell Experiment Protocol
- Troubleshooting
- Therapeutic mAbs & Proteins
-
Vaccine Development
- Adenovirus Vaccines
- Development of Chikungunya Virus Vaccines
- Development of Clostridioides difficile Vaccine
- Development of Cytomegalovirus (HHV-5) Vaccines
- Development of Ebola Virus Vaccines
- Development of Epstein–Barr Virus Vaccine
- Development of Human Immunodeficiency Virus (HIV) Vaccine
- Development of Human Metapneumovirus Vaccine
- Development of Klebsiella Pneumoniae Vaccines
- Development of M. tuberculosis Vaccines
- Development of Neisseria Gonorrhoeae Vaccines
- Development of Nipah Virus Vaccine
- Development of Norovirus Vaccines
- Development of Parainfluenza Virus Type 3 Vaccine
- Development of Rhinovirus Vaccines
- Development of Shigella Vaccines
- Development of Smallpox Virus Vaccines
- Development of Varicella zoster virus Vaccines
- Development of Venezuelan Equine Encephalitis Virus Vaccines
- Development of Zika Virus Vaccine
- DTP Vaccine
- Enterovirus 71 Vaccines and Prevention of Hand-foot-mouth Disease (HFMD)
- Haemophilus influenzae Type b (Hib) Vaccine
- Hepatitis Vaccine
- HPV Vaccine
- Influenza Vaccine
- Listeria monocytogenes-Based Vaccines
- Lyme Disease Vaccine
- Malaria Vaccines
- Measles-Mumps-Rubella (MMR) Vaccine
- Meningococcal Vaccine
- Monkeypox Virus Vaccines
- mRNA Vaccine
- Pneumococcal Disease
- Polio Vaccine
- Rabies Virus Vaccines
- Respiratory Syncytial Virus Vaccines
- S. pneumoniae Vaccine
- Research Review
Page Content
Ordering Information
Payment methods we support:
Invoice / Purchase Order
Credit card
Invoice / Purchase Order
Credit card
