Anti-CDKN1A monoclonal antibody
Mouse anti-Human CDKN1A monoclonal antibody for IHC
COA
Specifications
Antibody Isotype
IgG2a
Clone
EDT-60.3
Species Reactivity
Human
Immunogen
BALB/C mice were injected with a full length human recombinant p21 protein.
Conjugate
Unconjugated
Images
IHC-P analysis of esophagus squamous cell carcinoma tissue by CDKN1A antibody.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded esophagus squamous cell carcinoma tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with CDKN1A antibody at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Tumor cells are positively stained at the nuclei.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded esophagus squamous cell carcinoma tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with CDKN1A antibody at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Tumor cells are positively stained at the nuclei.
Target
Alternative Names
CDKN1A; cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CAP20; CDKN1; CDKN1A; CIP1; P21; p21; PIC1; p21 / WAF1; p21Cip1/Waf1; p21CIP1; SDI1; WAF1; MDA-6
Entrez Gene ID
UniProt ID
Product Background
Antigen Description
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair.
Pathway
AKT phosphorylates targets in the cytosol; AMPK signaling; Adipogenesis; Alpha6-Beta4 Integrin Signaling Pathway; Bladder cancer; C-MYB transcription factor network; Cell Cycle Checkpoints; Cell Cycle, Mitotic; Cell cycle; Chronic myeloid leukemia; Class
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
Citations
Have you cited DMAB5419MH in a publication? Let us know and earn a reward for your research.
| Product Name | Cat. No. | Applications | Host Species | Datasheet | Price | Add to Basket |
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Camacho, H; Fernandez, ME; et al. Analysis of cell proliferation and gene expression profiles in Epidermal Growth Factor-treated tumor cell lines. MINERVA BIOTECNOLOGICA 25:43-54(2013).
Nishimura, J; Dewa, Y; et al. Possible involvement of oxidative stress in fenofibrate-induced hepatocarcinogenesis in rats. ARCHIVES OF TOXICOLOGY 82:641-654(2008).
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Catalog # DMAB5419MH
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