Anti-TG monoclonal antibody

Autoantibodies against thyroid peroxidase (TPO); described initially as thyroid microsomal antigen, are found in most of the patients with autoimmune thyroid disorders. The present study comprised of development, optimization and validation of 'sandwich' radioassay for the measurement of TPOAb in human serum. The standardized TPOAb radioassay has maximum binding of 25-30%, non-specific binding of < 0.4%, analytical sensitivity of approximately around 1 IU/ml, acceptable intra- and inter-assay %CV, with a standard working range up to 1400 IU/ml. The in-house developed assay is simple, robust and cost-effective, and therefore can be used for routine measurement of TPOAb in clinical samples.

Thyroid cancer (TC) is the most prevalent malignant neoplasm that affects the endocrine system. Hashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is the most common autoimmune thyroid ease (AITD) that, together with Graves' disease (GD), represent the main autoimmune diseases that affect thyroid gland. Some studies suggest a greater risk of AITD and the development of TC, while others, investigate its relationship with TC progression and patient prognosis. In this review, we have analyzed published data the molecular aspects related to the association between AITD and TC, addressing their influence on TC gression, diagnosis, and prognosis of the patients. MEDLINE database (PubMed) platform was used as a search engine and the original articles related to the topic were selected using the keywords combination "thyroid cancer and Hashimoto thyroiditis" or "thyroid carcinoma and thyroid autoimmune disease". After the selection, we categorized the main findings of the papers into four topics: antitumor immunity, tumor progression, agnosis, and prognosis. Although most of the studies have pointed out the presence of AITD as a factor increases the risk of TC, few molecular mechanisms to support this conclusion have been described. Additionally, little information is available to explain, pathophysiologically, the effects of autoimmunity in TC diagnosis, progression, and prognosis.