Intended Use
Quantitative determination of Human Bcl-2
Detection Range
Assay range: 78.13-5000 pg/mL
General Description
ABPR-ZB006 is a solid phase sandwich ELISA for quantitative determination of Human Bcl-2.
Reconstitution And Storage
Reconstitution
1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating.
2. Detection Antibbody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 mL detection antibody dilution buffer.
Storage
1. Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
2. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
3. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Citations
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Liu, Y; Yang, H; et al. AGGF1 protects from myocardial ischemia/reperfusion injury by regulating myocardial apoptosis and angiogenesis. APOPTOSIS 19:1254-1268(2014).
Zent, CS; Burack, WR; et al. Mutations in chronic lymphocytic leukemia and how they affect therapy choice: focus on NOTCH1, SF3B1, and TP53. HEMATOLOGY-AMERICAN SOCIETY OF HEMATOLOGY EDUCATION PROGRAM :119-124(2014).