Intended Use
Quantitative determination of Human BID
Detection Range
Assay range: 7.81-500 pg/mL
General Description
ABPR-ZB296 is a solid phase sandwich ELISA for quantitative determination of Human BID.
Reconstitution And Storage
Reconstitution
1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating.
2. Detection Antibbody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 mL detection antibody dilution buffer.
Storage
1. Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
2. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
3. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Citations
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Aoudjehane, L; Podevin, P; et al. Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway. FASEB JOURNAL 21:1433-1444(2007).
Fujino, M; Li, XK; et al. Distinct pathways of apoptosis triggered by FTY720, etoposide, and anti-Fas antibody in human T-lymphoma cell line (Jurkat cells). JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 300:939-945(2002).