Carnation mottle virus (CarMV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect CarMV in infected carnation plant.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is super high. The virus can be consistently detected in infected plant tissues diluted at 1:2430 - 1:7290.

General Description

Carnation mottle virus (CarMV) is a plant pathogenic virus of the family Tombusviridae. The origin of Carnation mottle virus (CarMV) is unknown. The virus was first detected in the UK, into which it was probably introduced in commercial stocks of carnations and/or pinks. It was described by Kassanis (1955) and Hollings & Stone (1970).
CarMV is an RNA-containing virus with isometric particles about 32-35 nm in diameter that mainly infects species in the Caryophyllaceae. It is transmitted mechanically but has no known vector. The virus is the type species of the genus Carmovirus which comprises 14 definitive and 8 tentative species and, together with seven additional genera (Tombusvirus, Dianthovirus, Aureusvirus, Avenavirus, Necrovirus, Panicovirus and Machlomovirus), is included in the family Tombusviridae (Lommel et al., 2005). The generic name is derived from the name of the type species, CARnation MOttle VIRUS.
Depending on cultivar and/or environmental conditions, typical strains of the virus induce mild mottling in the young leaves and faint chlorosis in mature leaves of carnations (Dianthus caryophyllus) and pinks (Dianthus barbatus). In some carnation cultivars, the quality and quantity of cut flowers is reduced (Calderon & Arbelaez, 1999). In Begonia elatior and Begonia × cheimantha CarMV induces vein clearing and curling of leaves and flower breaking (Anonymous, 1984; Paludan & Begtrup, 1985). The leaves of Calla lily (Zantedeschia spp.) develop yellow mottling, yellow ringspot and a general mosaic (Chen et al., 2003b). The virus occurs symptomlessly in infected lettuce (Lactuca sativa) and Daphne × burkwoodii and Daphne odora (Tomlinson & Faithfull, 1976; Morris-Krsinich & Milne, 1977).

Standard Curve