Intended Use
Quantitative determination of Human MCP-1/CCL2
Detection Range
Assay range: 3.91-250 pg/mL
General Description
ABPR-ZB275 is a solid phase sandwich ELISA for quantitative determination of Human MCP-1/CCL2.
Reconstitution And Storage
Reconstitution
1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating.
2. Detection Antibbody: Dilute to working concentration of 0.5 μg/mL in Dilution Buffer before use.
3. Standard: Reconstitute with 1 mL Dilution Buffer.
Storage
1. Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
2. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
3. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Citations
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Ali, TH; Pisanti, S; et al. Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumour-infiltrated lymph nodes of melanoma patients. NATURE COMMUNICATIONS 5:-(2014).
Gobbetti, T; Coldewey, SM; et al. Nonredundant protective properties of FPR2/ALX in polymicrobial murine sepsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111:18685-18690(2014).