p21 Cip1 (Phospho-Thr145) ELISA Kit

Sample

cultured cells

Species Reactivity

Human, Mouse, Rat

Intended Use

The p21 Cip1 (Phospho-Thr145) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p21 Cip1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated p21 Cip1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p21 Cip1 phosphorylation.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-p21 Cip1 (Phospho-Thr145) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-p21 Cip1 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x), Clear
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x), Clear
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x), Clear
16. Adhesive Plate Seals: 4 Seals

Storage

4°C/6 Months