Sample
serum, plasma, cell culture supernatants, tissue homogenate
Intended Use
For quantitative detection of Human IFNAR1 (Interferon alpha/beta receptor 1) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Citations
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Croze, E; RussellHarde, D; et al. The human type I interferon receptor - Identification of the interferon beta-specific receptor-associated phosphoprotein. JOURNAL OF BIOLOGICAL CHEMISTRY 271:33165-33168(1996).
Krishnan, K; Yan, H; et al. Dimerization of a chimeric CD4-interferon-alpha receptor reconstitutes the signaling events preceding STAT phosphorylation. ONCOGENE 13:125-133(1996).