Human Neurofilament Protein [His]

Product Overview

NEFL Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (2-543 a.a) containing 551 amino acids including a 9 a.a N-terminal His tag.

Nature

Recombinant

Tag/Conjugate

His

Molecular Weight

The total molecular mass is 62.5kDa (calculated).

Alternative Names

NEFL; Neurofilament light polypeptide; NFL; NF-L; NF68; 68 kDa neurofilament protein

Purity

Greater than 90.0% as determined by SDS-PAGE.

Format

Lyophilized

Concentration

It is recommended to add 200 ul of deionized waterto prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.

Buffer

Lyophilized from 0.5mg/ml solution in 15mM Tris and 85mM Glycine, pH 8.5.

Preservative

None

Storage

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Introduction

NEFL or Neurofilament light polypeptide is a protein that is encoded through the NEFL gene. NEFL is correlated to a disease called Charcot–Marie–Tooth. The protein's light subunit is determined by immunoassays in the plasma and cerebrospinal fluid, if present, it can indicate on axonal damage in neurological diseases. By doing so, NEFL can act as a marker for Huntington's disease, Amyotrophic Lateral Sclerosis and multiple sclerosis.

Keywords

NEFL; Neurofilament light polypeptide; NFL; NF-L; NF68; 68 kDa neurofilament protein