Human T3 (Total) ELISA Kit

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

COA

SDS

Datasheet

Sample

Serum

Species Reactivity

Human

Intended Use

The Human T3 (Total) ELISA Kit is intended for the quantitative determination of triiodothyronine(T3) concentration in human serum.

Contents of Kit

1. Goat Anti-Mouse IgG Coated Microtiter Wells, 96 wells. Ready to use. 1
2. Enzyme Conjugate Concentrate (11×). Concentrated. 1.3 mL
3. Enzyme Conjugate Diluent. Ready to use. 13 mL
4. T3 Reference Standard, 0, 0.75, 1.5, 3.0, 6.0 and 10.0ng/ mL. Ready to use. 1 set, 1.0 mL each
5. Antibody Reagent. Ready to use. 7 mL
6. TMB Reagent (One-Step). Ready to use. 11 mL
7. Stop Solution(1N HCl). Ready to use. 11 mL

Storage

Unopened test kitsshould be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above.

Precision

Intra-Assay: 5.63%
Inter-Assay: 4.96%

Detection Range

0 to 10 ng/mL

Sensitivity

The minimum detectable concentration of T3 by this assay is estimated to be 0.2 ng/ml.

General Description

The thyroid gland exerts powerful and essential regulatory influences on growth, differentiation, cellular metabolism, and general hormonal balance, as well as on the maintenance of metabolic activity and the development of the skeletal and organ system. Thehormonesthyroxine(T4) and 3,5,3'triiodothyronine(T3) circulate in the blood stream, mostly bound to the plasma protein, thyroxinebindingglobulin(TBG). The concentration of T3 is much less than that of T4, but its metabolic potency is much greater. T3 determination is an important factor in the diagnosis of thyroid disease. Its measurement has uncovered a variant of hyperthyroidism in thyrotoxic patients with elevated T3 levels and normal T4 levels. An increase in T3 without an increase in T4 is frequently a forerunner of recurrent thyrotoxicosis in previously treated patients. In other patients, euthyroidism is attributable to normal T3, although their T4 values are subnormal. T3 determination is also useful in monitoring both patients under treatment for hyperthyroidism and patients who have discontinued anti-thyroid drug therapy. It is especially valuable in distinguishing between euthyroid and hyperthyroid subjects. In women, T3 levels are elevated during pregnancy, during estrogen treatment, and contraceptive hormone therapy.When T3 levelsparallel TBG increases in a manner analogous to T4 levels, these changes are not a reflection of altered thyroid status.

Standard Curve

Results of a typical standard run with optical density readings at 450 nm shown on the Y-axis against Total T3 concentrations shown in the X-axis. This standard curve is for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain his or her own data and standard curve.

Citations

Have you cited DEIA4245 in a publication? Let us know and earn a reward for your research.

Product Name	Cat. No.	Applications	Host Species	Datasheet	Price	Add to Basket

Product Name	Cat. No.	Applications	Host Species	Datasheet	Price	Add to Basket

Datasheet

Certificate of Analysis

Safety Data Sheet

Customer Reviews

Write a review

A sensitive ultra-fast liquid chromatography with tandem mass spectrometrymethod for simultaneous determination of ten constituents of Deng-Zhan-Xi-Xin injection in rat plasma and its application to a comparative pharmacokinetic study in sham and middle cerebral artery occlusion rats

JOURNAL OF SEPARATION SCIENCE

Authors: Guo, Xin; Lin, Shan; Liang, Wei; Dou, Xiuxiu; Wang, Xinyu; Tian, Xinhui

Abstract

Deng-Zhan-Xi-Xin injection is widely used to treat cerebrovascular and cardiovascular diseases in clinical practice. A rapid and selective method based on ultra-fast liquid chromatography with tandem mass spectrometry was established and validated to simultaneously quantify chlorogenic acid, 1,3-O-dicaffeoylquinic acid, isochlorogenic acid A, neochlorogenic acid, erigeside I, cryptochlorogenic acid, apigenin-7-O-glucuronide, scutellarin, isochlorogenic acid B, and isochlorogenic acid C of Deng-Zhan-Xi-Xin injection in both sham and middle cerebral artery occlusion rats. This was the first quantitative analysis of these ten constituents in both sham and middle cerebral artery occlusion rats. Chromatographic separation of these ten constituents was accomplished on an Acquity HSS T3 column with the mobile phase consisting of acetonitrile and 0.1% formic acid in water. Mass analysis was performed in negative ion mode with an electrospray ionization source using multiple reaction monitoring technology. The pharmacokinetic study of the ten constituents in sham and middle cerebral artery occlusion rats after intravenous administration of Deng-ZhanXi-Xin injection was successfully accomplished by using this validated method. Based on the results of pharmacokinetic parameters, significant differenceswere observed between the two groups, whichmight be due to the pathological factors of middle cerebral artery occlusion and pharmacological effects of Deng-ZhanXi-Xin injection.

Cdc-Like Kinases (CLKs): Biology, Chemical Probes, and Therapeutic Potential

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Authors: Martin Moyano, Paula; Nemec, Vaclav; Paruch, Kamil

Abstract

Protein kinases represent a very pharmacologically attractive class of targets; however, some members of the family still remain rather unexplored. The biology and therapeutic potential of cdc-like kinases (CLKs) have been explored mainly over the last decade and the first CLK inhibitor, compound SM08502, entered clinical trials only recently. This review summarizes the biological roles and therapeutic potential of CLKs and their heretofore published small-molecule inhibitors, with a focus on the compounds' potential to be utilized as quality chemical biology probes.