ABPR-ZB072 is a solid phase sandwich ELISA for quantitative determination of Human IL-6.
Reconstitution And Storage
Reconstitution 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibbody: Dilute to working concentration of 0.25 μg/mL in Dilution Buffer before use. 3. Standard: Reconstitute with 1 mL Dilution Buffer. Storage 1. Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. 2. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! 3. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Citations
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Human IL-6 Antibody Pair Set
The minimum detectable dose of Human IL-6/Interleukin-6 was determined to be approximately 3.13 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
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