Anti-Human IL6 Monoclonal Antibody
Mouse Anti-Human IL6 Monoclonal Antibody for IHC, WB
COA
Specifications
Antibody Isotype
IgG1
Clone
20
Species Reactivity
Human
Immunogen
Recombinant human IL-6 protein
Conjugate
Unconjugated
Applications
Application Notes
IHC, WB
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Images
IHC-P analysis of appendix tissue by IL6 antibody.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded appendix tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with IL6 antibody at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in lamina propria are positively stained at the cytoplasm.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded appendix tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with IL6 antibody at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in lamina propria are positively stained at the cytoplasm.
Target
Alternative Names
IL6; Interleukin-6; BSF2; HSF; IFNB2
UniProt ID
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
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Catalog # DMABB-JX195
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