There is also a link between APOE and DN. Diabetic Nephropathy is a common chronic complications of diabetes. According to the research, these patients with DN have an
obvious reduction of APOE. The reason is that kidney is one of the sources of APOE and damage of kidney leads to the decline of APOE serum. So APOE test is significant to diagnosis of related disease. The tests are mainly divided into two kinds of methods: the methods to test genotype of APOE and the test of concentration of APOE.

Test of APOE’s Genotype
Genotype test is use PCR to enlarge genome sequence of APOE and then test its polymorphism in gene level. Separate polyacrylamide gel electrophoresis from enzyme and mutation – specific amplification system will use the specific Oligonucleotide as one of primers of PCR. If there is mutation, it will have PCR action. If it is not, it has action with normal primers. Polymorphism analysis of single strand conformation depends on that DNA of polymorphism have sequence-specific conformation in polypropylene non-denaturing gel electrophoresis. APOE’s polymorphism is related to many disease, so rapid and right test of APOE can provide evidences of diagnosis and treatment of many disease.

Test of Concentration of APOE
The earliest way to test the concentration of APOE is radio immunoassay. But it keeps difference due to the difference of reagents,samples,process in different labs. APOE Elisa, Immunoelectrophoresis and Immunoturbidimetric are also important ways to test APOE. All of these methods have variation for the different condition. RIA and APOE Elisa are more sensitive than others.No matter what methods are used to test, the appropriate result must be produced in the standard assays. The effected factors of APOE’s concentration of blood concludes gender, age and smoking history. The concentration will reduce since people was born and will increase after they are thirty years old. Then it will reduce again after they are sixty years old. About 80% of smokers have a rising of APOE in blood. People with Hypertriglyceridemia have a higher concentration of APOE than people with high cholesterol. Patients with Coronary Heart Disease have a obvious change of APOE.

That is the test methods.

The post How to Test APOE first appeared on Creative Diagnostics.

So testing HP plays a vital role in curing gastric disease. As technology and medicine advance, there are a variety of methods to testing HP.

First, C14 breathing test. HP is the only bacterium in stomach which can produce plenty of Urease, so Urease can test whether a patient was infected or not. This test is regarded as the most convenient and accurate way to test HP.

Second, biopsy. Clip disease tissue to do pathological examination when a patient do gastroscopy. If it is positive, it means infection.

Third, blood test. It test the HP antibody in serum to test the HP. This test includes complement fixation test, agglutination test, passive hemagglutination assay, immunoblotting and combined enzyme assay. Antibody test is an important part of blood test. It utilize the colloidal gold to test detect HP antibody in serum and plasma. The test area covered with nitrocellulose film have HP antibody and QC area is marked with HP polyclonal antibody. Glass fiber paper in the bottom of sample is fixed with colloidal gold particles of H pylori antigen. This is the test process: first, add sample into the colloidal gold particles and membrane will move to test are because of capillary action mixture. When the sample of the patient have HP specific antibody, it will have an action with antigen of test line and form a mixture of antigen,antibody and antigen colloidal gold particles, then a red line shows. On the contrary, test are having no presence of color means negative.

These are the main methods to test HP. It is advised to have a regular test of HP to prevent gastric disease from you.

The post How to Test Helicobacter Pylori first appeared on Creative Diagnostics.

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Colorectal Cancer is the tumor with high prevalence rate and the mortality ranks the second among Common Malignancies in recent years. The incidence of Colorectal Cancer have shown a obvious upward trends. So it is urgent to use FOB to diagnose it in early stage to reduce the mortality. Fecal Occult Blood Rapid Test is regarded as the world-recognized screening method to Colorectal Cancer. Because most pathological changes have the presence of bleeding, it can give a clear signal. However, a large number of people do not take this test seriously, which always causes the delay of treatment.

Generally, the main symptom of Colorectal Cancer is hematochezia and other symptoms are diabetes, stomach ache, weight loss and Anemia. What needs to be noticed is that some Colorectal Cancer in early stage shows no clear symptoms, so patients fail to recognize hematochezia by themselves. So it is necessary to do the regular stool test. It is late to do the test when they have stomach ache, diabetes or see blood in stool. In addition, the patients of Colorectal Cancer become younger. According to some medical papers, young people with Colorectal Cancer have bad changes and cancer cells spread in a quicker speed. Sometimes, people think having difficulty in defecation as constipation and they do not choose to have a test. Thus it will leads irreparable loss.

Therefore, it is significant to do Fecal Occult Blood Rapid Test（FOB）to save patients’ life. It is known that the test is regarded as an important screening index in diagnosing digestive tract cancer. The accuracy of the stool test is the important reason. About 20 percent of patients having diagnose digestive tract cancer in early stage is positive in the test and over 90 percent of patients with advanced cancer are positive in the test.

So, to get regular Fecal Occult Blood Rapid Test（FOB）in hospital having high-quality stool test kits to avoid irreparable loss.

The post World-recognized Diagnostics Method of Colorectal Cancer—FOB first appeared on Creative Diagnostics.