Intended Use
Quantitative determination of Human MMP-9
Detection Range
Assay range: 125-8000 pg/mL
General Description
ABPR-ZB343 is a solid phase sandwich ELISA for quantitative determination of Human MMP-9.
Reconstitution And Storage
Reconstitution
1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in PBS before coating.
2. Detection Antibbody: Dilute to working concentration of 0.1 μg/mL in Dilution Buffer before use.
3. Standard: Reconstitute with 1 mL Dilution Buffer.
Storage
1. Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
2. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
3. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Citations
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Visvanathan, S; Wagner, C; et al. E-selectin, Interleukin 18, Serum Amyloid A, and Matrix Metalloproteinase 9 Are Associated with Clinical Response to Golimumab plus Methotrexate in Patients with Active Rheumatoid Arthritis Despite Methotrexate Therapy. JOURNAL OF RHEUMATOLOGY 36:1371-1379(2009).
Brule, S; Friand, V; et al. Glycosaminoglycans and syndecan-4 are involved in SDF-1/CXCL12-mediated invasion of human epitheloid carcinoma HeLa cells. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1790:1643-1650(2009).