Sample
serum, plasma, cell culture supernatants, tissue homogenate
Intended Use
For quantitative detection of Mouse Pou5f1 ( POU domain, class 5, transcription factor 1) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Citations
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Jones, TD; Ulbright, TM; et al. OCT4 staining in testicular tumors - A sensitive and specific marker for seminoma and embryonal carcinoma. AMERICAN JOURNAL OF SURGICAL PATHOLOGY 28:935-940(2004).
Katoh, M; Katoh, M; et al. Integrative genomic analyses of CXCR4: Transcriptional regulation of CXCR4 based on TGF beta, Nodal, Activin signaling and POU5F1, FOXA2, FOXC2, FOXH1, SOX17, and GFI1 transcription factors. INTERNATIONAL JOURNAL OF ONCOLOGY 36:415-420(2010).