Rat TSH ELISA Kit

Sample

serum

Species Reactivity

Rat

Intended Use

The TSH rat ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research use only. Not for use in diagnostic procedures.

Storage

When stored at 2 - 8 °C unopened reagents will be stable until expiration date. Do not use reagents beyond this date. Opened reagents must be stored at 2 - 8 °C. After first opening the reagents are stable for 30 days if used and stored properly.
Microtiter wells must be stored at 2 - 8 °C. Take care that the foil bag is sealed tightly.
Store Master Standard refrigerated, they will be stable at 2 - 8 °C for 7 days after reconstitution or until expiration date. For longer storage freeze at -20 °C.

Precision

The intra-assay variation was determined by 20 replicate measurements of 3 samples within one run. The within-assay variability is shown below:

The inter-assay (between-run) variation of 3 serum samples was determined in 9 different assays.

Sensitivity

The lowest analytical detectable level of TSH that can be distinguished from the Standard A is 0.081 ng/ml at the 2SD confidence limit.

General Description

Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein produced by the anterior pituitary gland. Through its action on the thyroid gland, it plays a major role in maintaining normal circulating levels of the iodothyronines, T4 and T3. The production and secretion of TSH is controlled on the one side by negative feedback from circulating T4 and T3, and on the other side by the hypothalamic thyrotropin-releasing hormone (TRH).
The TSH molecule is composed of two non-identical subunits, α and β, that are bound together in a noncovalent manner. Within a species, the TSH α unit is structurally identical to the α subunits of related glycoprotein hormones (LH, FSH). The β subunits of the related hormones are structurally hormone-specific and therefore determine their unique biological activities.
The mechanism controlling thyroid function in rats is exactly analogous to the mechanism operating in humans. This means that thyrotropin-releasing hormone stimulates the release of TSH from the pituitary gland as well as the serum concentrations of T4 and T3 influence the action of the pituitary gland. This similarity between rat and human thyroid physiology makes the rat a very useful model for evaluating the effects of new drugs on thyrometabolic status.