Human IL8 peptide

Recombinant Human IL8 peptide

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Product Overview

Recombinant Human Interleukin-8 (IL-8) (amino acids 1-77) RecombinantHuman IL-8 is a single, non-glycosylated polypeptide chain containing 77amino acids and having a molecular weight of 8,904 Da. The sequence of thefirst five N-terminal amino acids was de

Nature

Recombinant

Tag/Conjugate

Unconjugated

Alternative Names

NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1

Format

Purified, Lyophilized. Reconstitute using sterile deionized water to a concentration ≥100μg/ml.Further dilutions can be made in other aqueou buffers.

Concentration

1 mg/ml(OD280nm, E0.1% = 0.85) (prior to lyophilization)

Buffer

Lyophilized fromwater containing no additives.

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

UniProt ID

3576

Antigen Description

Interleukin-8 (IL-8) is a chemokine produced by macrophagesand other cell types such as epithelial cells. It is also synthesized byendothelial cells, which store IL-8 in their storage vesicles, theWeibel-Palade bodies. In humans, the interleukin-8 protein is encoded by theIL8 gene. There are more receptors of the surface membrane capable to bindIL-8; the most frequently studied types are the G protein coupled serpentinereceptors CXCR1 and CXCR2. Expression and affinity to IL-8 is different inthe two receptors (CXCR1; CXCR2). Toll-like receptors are the receptorsof the innate immune system.

Keywords

IL8; interleukin 8; interleukin-8; IL-8; C-X-C motif chemokine 8;

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Post-stroke executive dysfunction and verbal fluency negatively correlated to IL8

EGYPTIAN JOURNAL OF NEUROLOGY PSYCHIATRY AND NEUROSURGERY

Authors: Shaheen, Hala A.; Daker, Lamiaa, I; Abbass, Mohammed M.; Abd El Fattah, Asmaa A.

Abstract

Background: Patients with acute ischemic stroke are at a higher risk of developing cognitive impairment which could be often attributed to cytokine activation. Objectives: To explore the relationship between the cognitive performance and the inflammatory markers in the ischemic stroke patients at the early stage. Patients and methods: A cross-sectional case-control study was performed on 44 ischemic stroke patients. The patients underwent the following battery of evaluation: (A) assessment of stroke disability by modified Rankin Scale (mRS) and National Institute of Health Stroke Scale (NIHSS). (B) neuropsychological evaluation using Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA) scale, trail making test (TMT), and Controlled Oral Word Association Test (COWAT). (C) Assessment of depression was done by using Beck Depression Inventory (BDI). Measurement of the serum levels of ESR, C-reactive protein, and IL-8 was done. This study included 44 ages, sex, and educational level matched controls for comparison of neuropsychological tests and serum level of IL-8. Results: The patients showed worse performance in neuropsychological tests (MMSE, MoCA, COWAT, TMT) than the controls. There was a significant negative correlation between the serum level of IL-8 and (MoCA) (r = -0.43, p = 0.004), verbal fluency (r = -0.56, p < 0.001), and positive significant correlation between IL8 and executive functions (r = 0.61, p < 0.001). Conclusion: The cognitive impairment in early acute ischemic stroke is highly correlated to the serum level of IL-8.

Cooperation between SS18-SSX1 and miR-214 in Synovial Sarcoma Development and Progression

CANCERS

Authors: Tanaka, Miwa; Homme, Mizuki; Yamazaki, Yukari; Ae, Keisuke; Matsumoto, Seiichi; Subramanian, Subbaya; Nakamura, Takuro

Abstract

SS18-SSX fusion proteins play a central role in synovial sarcoma development, although, the genetic network and mechanisms of synovial sarcomagenesis remain unknown. We established a new ex vivo synovial sarcoma mouse model through retroviral-mediated gene transfer of SS18-SSX1 into mouse embryonic mesenchymal cells followed by subcutaneous transplantation into nude mice. This approach successfully induced subcutaneous tumors in 100% recipients, showing invasive proliferation of short spindle tumor cells with occasional biphasic appearance. Cytokeratin expression was observed in epithelial components in tumors and expression of TLE1 and BCL2 was also shown. Gene expression profiling indicated SWI/SNF pathway modulation by SS18-SSX1 introduction into mesenchymal cells and Tle1 and Atf2 upregulation in tumors. These findings indicate that the model exhibits phenotypes typical of human synovial sarcoma. Retroviral tagging of the tumor identified 15 common retroviral integration sites within the Dnm3 locus as the most frequent in 30 mouse synovial sarcomas. miR-199a2 and miR-214 upregulation within the Dnm3 locus was observed. SS18-SSX1 and miR-214 cointroduction accelerated sarcoma onset, indicating that miR-214 is a cooperative oncomiR in synovial sarcomagenesis. miR-214 functions in a cell non-autonomous manner, promoting cytokine gene expression (e.g., Cxcl15/IL8). Our results emphasize the role of miR-214 in tumor development and disease progression.