SARS S1 protein [His]

Product Overview

SARS S1 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1). This protein carries a polyhistidine tag at the C-terminus.

Nature

Recombinant

Predicted N terminal

Ser 14

Tag/Conjugate

His

Molecular Weight

The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Alternative Names

SARS; coronavirus; SARS S1; SARS spike protein

Purity

>95% as determined by SDS-PAGE.

Format

Lyophilized

Buffer

Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Preservative

None

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Introduction

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Keywords

SARS; coronavirus; SARS S1; SARS spike protein