SARS Spike Protein (Receptor Binding Domain) [Fc]

Product Overview

A DNA sequence encoding the receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with the Fc region of rabbit IgG at the C-terminus.

Nature

Recombinant

Predicted N terminal

Arg 306

Tag/Conjugate

Fc

Molecular Weight

The recombinant receptor binding domain (RBD) of human SARScoronavirus (isolate:WH20) spike comprises 446 amino acids and has a predicted molecular mass of 50.2 kDa. The apparent molecular mass of the protein is approximately 78.9 and 54.3 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1 .0 EU per μg of the protein as determined by the LAL method

Purity

(36.8+ 56.0) % as determined by SDS-PAGE

Format

Lyophilized

Concentration

Specific concentrations are included in the hardcopy of COA.

Buffer

Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 8.0.

Preservative

None

Storage

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Stability

Samples are stable for up to twelve months from date of receipt at -70 ℃

Introduction

SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. The E.Coli derived 38 kDa mosaic protein contains the N-terminal section of the Spike protein.