Sample
serum, plasma, cell culture supernatant and other biological samples
Intended Use
In vitro quantitative determination of T3 concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date
Performance Characteristics
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
0.078-5ng/ml
General Description
T3 (Triiodothyronine) is a hormone synthesized and secreted by the thyroid gland, and it is also a type of thyroid hormone. T3 is a biologically active hormone that can affect various processes in the body such as metabolism, growth, and development. It can promote protein synthesis, bone growth and maturation, stimulate the development and maturation of the central nervous system, promote the normal functioning of the cardiovascular system, and regulate body temperature, heart rate, and muscle function. The production of T3 mainly relies on the conversion of the thyroid hormone precursor T4, and most of the T3 is produced by the deiodination of T4 in various tissues and organs. T3 has a wide range of effects on the human body, and both excessive and insufficient secretion of T3 by the thyroid gland can have negative impacts on normal body functions, leading to diseases such as hyperthyroidism and hypothyroidism.
Standard Curve
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
![](http://img2.creative-diagnostics.com/productimages/DEIA-NS2404-3-4.png)
Citations
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Xia, T; Zhang, Q; et al. CREB/TRH pathway in the central nervous system regulates energy expenditure in response to deprivation of an essential amino acid. INTERNATIONAL JOURNAL OF OBESITY 39:105-113(2015).
Chand, N; Naz, S; et al. Performance traits and immune response of broiler chicks treated with zinc and ascorbic acid supplementation during cyclic heat stress. INTERNATIONAL JOURNAL OF BIOMETEOROLOGY 58:2153-2157(2014).