Sample
serum, plasma, cell culture supernates, tissue homogenates
Intended Use
For the quantitative determination of endogenic rat thyroxine (T4) concentrations in serum, plasma, cell culture supernates, tissue homogenates, cell lysates.
Contents of Kit
Assay plate: 1(96 wells)
Standard: 5 x 1 ml
Antibody: 1 x 6 ml
HRP-conjugate: 1 x 6 ml
Wash Buffer (20 x concentrate): 1 x 15 ml
Substrate A: 1 x 7 ml
Substrate B: 1 x 7 ml
Stop Solution: 1 x 7 ml
Adhesive Strip (For 96 wells): 4
Instruction manual:1
Storage
Unopened kit: Store at 2 - 8°C. Do not use the kit beyond the expiration date.
Opened kit: May be stored for up to 1 month at 2 - 8°C.
Precision
Intra-assay Precision (n=3): CV%<15%
Inter-assay Precision (n=3): CV%<15%
Detection Range
20 ng/ml-320 ng/ml.
Sensitivity
The minimum detectable dose of rat T4 is typically less than 20 ng/ml.
Citations
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Schuerwegh, AJ; Verhelst, J; et al. Wegener's granulomatosis presenting as a thyroid mass. CLINICAL RHEUMATOLOGY 26:454-456(2007).
Eskes, SA; Endert, E; et al. Selenite supplementation in euthyroid subjects with thyroid peroxidase antibodies. CLINICAL ENDOCRINOLOGY 80:444-451(2014).