Intended Use
Quantitative determination of Human TNF-alpha
Detection Range
Assay range: 23.44-1500 pg/mL
General Description
ABPR-ZB157 is a solid phase sandwich ELISA for quantitative determination of Human TNF-alpha.
Reconstitution And Storage
Reconstitution
1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating.
2. Detection Antibbody: Dilute to working concentration of 0.5 μg/mL in Dilution Buffer before use.
3. Standard: Reconstitute with 1 mL Dilution Buffer.
Storage
1. Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
2. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
3. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Citations
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Cohen, N; Sabhachandani, P; et al. Approaching near real-time biosensing: Microfluidic microsphere based biosensor for real-time analyte detection. BIOSENSORS & BIOELECTRONICS 66:454-460(2015).
Patterson, JL; Arenas-Gamboa, AM; et al. Materials composed of the Drosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103:1546-1553(2015).