Intended Use
Quantitative determination of Human TNF beta
Detection Range
Assay range: 6.25-400 pg/mL
General Description
ABPR-ZB091 is a solid phase sandwich ELISA for quantitative determination of Human TNF beta.
Reconstitution And Storage
Reconstitution
1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating.
2. Detection Antibbody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 mL detection antibody dilution buffer.
Storage
1. Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
2. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
3. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Citations
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Shim, E; Babu, JP; et al. Glycated albumin produced in diabetic hyperglycemia promotes monocyte secretion of inflammatory cytokines and bacterial adherence to epithelial cells. JOURNAL OF PERIODONTAL RESEARCH 50:197-204(2015).
Cohen, N; Sabhachandani, P; et al. Approaching near real-time biosensing: Microfluidic microsphere based biosensor for real-time analyte detection. BIOSENSORS & BIOELECTRONICS 66:454-460(2015).