Species Reactivity
Human, Mouse
Intended Use
The p73 (Phospho-Tyr99) Colorimetric Cell-Based ELISA Kit allows for the qualitative detection of p73 (Phospho-Tyr99) and the effects that certain stimulation conditions have on p73 (Phospho-Tyr99) expression in different cell lines.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates.
2. 10x TBS: 24 mL (10x), Clear.
3. Quenching Buffer, 24 mL (1x), Clear.
4. Blocking Buffer, 50 mL (1x), Clear.
5. 10x Wash Buffer, 50 mL (10x), Clear.
6. 100x Anti-p73 (Phospho-Tyr99) Antibody (Rabbit Polyclonal) 60 μL (100x) Red.
7. 100x Anti-p73 Antibody (Rabbit Polyclonal) 60 μL (100x) Purple.
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 μL (100x) Green.
9. HRP-Conjugated Anti-Rabbit IgG Antibody, 12 mL (1x), Glass.
10. HRP-Conjugated Anti-Mouse IgG Antibody, 12 mL (1x), Glass.
11. Primary Antibody Diluent, 12 mL (1x), Clear.
12. Ready-to-Use Substrate, 12 mL (1x), Brown.
13. Stop Solution, 12 mL (1x), Clear.
14. Crystal Violet Solution, 12 mL (1x), Glass.
15. SDS Solution, 24 mL (1x), Clear.
16. Adhesive Plate Seals, 4 Seals.
Citations
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Holcakova, J; Ceskova, P; et al. The cell type-specific effect of TAp73 isoforms on the cell cycle and apoptosis. CELLULAR & MOLECULAR BIOLOGY LETTERS 13:404-420(2008).
Nenutil, R; Ceskova, P; et al. Differential expression of p73 alpha in normal ectocervical epithelium, cervical intraepithelial neoplasia, and invasive squamous cell carcinoma. INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY 22:386-392(2003).