Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
Our Antibody Labeling Services:
- Enzyme Labeling
- Biotin Streptavidin Labeling
- Fluorescence Labeling
- Nanoparticles Conjugation
- Oligonucleotide Conjugation
What's Included?
- Generation of conjugate at required scale from 100 ug to 1g
- Antibody clean up prior to conjugation (removal of BSA or azide)
- Antibody buffer exchanged into an optimized conjugation buffer
- Performed by conjugation professionals
- Evidence of successful conjugation provided
Benefits of This Service:
- Agreed conjugation ratio and scale
- Evidence of successful conjugation provided
- Reproducible
- Fast turnaround time
- Full technical support
Antibody conjugates are used in a large number of immunoassay applications including:
| Applications | Label |
| Western Blotting | • Enzymes (usually HRP or Alkaline Phosphatase) |
| ELISA | • Enzymes • Biotin/Streptavidin |
| Lateral Flow Immunoassay | • Fluorescent dyes • Gold Nanoparticles • Magnetic Nanoparticles • Quantum dots • Upconverting Nanoparticles |
| Immunofluorescence | • Fluorescent dyes |
| Immunohistochemistry | • Enzymes • Biotin/Streptavidin |
| Flow Cytometry | • Fluorescent proteins • Fluorescent dyes |
| ImmunoPCR | • Oligonucleotide |
Enzyme Labeling
- Alkaline Phosphatase (AP)
- Glucose Oxidase
- Horseradish Peroxidase (HRP)
Biotin Streptavidin Labeling
- Biotin
- Streptavidin
Fluorescence Labeling
| Fluorescence | Maximal Absorbance (nm) | Maximal Emission (nm) | |
| AMCA | 352 | 452 | |
| DyLight® 350 | 354 | 432 | |
| Atto 390 | 388 | 468 | |
| DyLight® 405 | 402 | 428 | |
| PerCP | 484 | 678 | |
| PerCP/Cy5.5 | 484 | 692 | |
| DyLight® 488 | 496 | 524 | |
| Fluorescein | 498 | 532 | |
| R-Phycoerythrin | 498,544,566* | 580 | |
| PE/Texas Red | 498,544,566* | 618 | |
| PE/Atto594 | 498,544,566* | 632 | |
| PE/Cy5 | 498,544,566* | 672 | |
| PE/Cy5.5 | 498,544,566* | 700 | |
| PE/Cy7 | 498,544,566* | 782 | |
| Alexa Fluor® 488 | 496 | 524 | |
| Atto 488 | 504 | 530 | |
| B-Phycoerythrin | 546 | 580 | |
| Cyanine Dye 3 (Cy3) | 552 | 576 | |
| Rhodamine | 555 | 588 | |
| DyLight® 550 | 556 | 584 | |
| Atto 565 | 570 | 598 | |
| DyLight® 594 | 594 | 629 | |
| Texas Red® | 596 | 616 | |
| DyLight® 633 | 628 | 660 | |
| Atto 633 | 634 | 660 | |
| FluoProbes647H | 650 | 684 | |
| Cyanine Dye 5 (Cy5) | 652 | 678 | |
| Allophycocyanin | 652 | 666 | |
| APC/Cy5.5 | 652 | 700 | |
| APC/Cy7 | 652 | 790 | |
| DyLight® 650 | 656 | 686 | |
| Cyanine Dye 5.5 (Cy5.5) | 680 | 705 | |
| DyLight® 680 | 686 | 716 | |
| Atto 700 | 704 | 724 | |
| DyLight® 755 | 756 | 794 | |
| DyLight® 800 | 776 | 798 |
*(R-)PE has three maximal asorbance, and all can be used. The optimal will depend on the application.
Nanoparticles Conjugation
- Gold nanoparticles
- Magnetic nanoparticles
- Upconverting nanoparticles
- Quantum dots
Oligonucleotide Conjugation
For us to better understand your customized project, please complete the Project Description part in the inquiry form. The more our scientists understand your project needs, the more accurate feedback we will be able to provide you. (the antibody you need, quantity, labeling, application)
Quotation and Ordering
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.