Monoclonal Antibody Production

Creative Diagnostics offer monoclonal antibody development services supported by a team with over 20 years of antibody development and manufacturing experience. We deliver a concierge service from project design through to delivery of your custom monoclonal antibody. Beginning with detailed discussions to understand your project goals, our technical team works with you to craft an antibody development plan that meets your needs.

Our Services of Monoclonal Antibody Development

Hybridoma Development Service

Hybridoma technology is the classical and economic technique used to produce monoclonal antibodies. The process includes the fusion of myeloma cells with B cells from immunized mice, the screening of antigen specific antibody-secreting hybridoma cells and obtaining monoclonal antibodies by production and purification.

• Our proprietary Magic™ mouse adjuvant can elicit stronger and faster immune response within only 21 days
• We fuse splenocytes with proper myeloma cells using optimized PEG mediated fusion
• Magic™ hybridoma growth factor successfully increase the cloning efficiency and raise the survival rate of hybridoma cell
• Customized hybridoma screening protocols according to the final applications of the requested antibodies, including but not limited to IP, WB, ELISA and IHC

Single B Cell Antibody Service

The single B cell antibody approaches harbor the potential to isolate functional mAbs reactive against conformational determinants that are present predominantly in vivo and difficult to emulate in vitro. We provide single B cell antibody service, involving single B cell isolation, sequencing, cloning and screening of single B cell antibodies.

• Available in our single B cell-based human, monkey, rabbit, mouse and rat monoclonal antibody discovery services
• Maximized chance to obtain functional & developable antibodies
• Cultured supernatant for high throughput FACS, WB or reporter cell assay etc. to help quickly narrow down positive candidates
• High efficiency in obtaining specific mAb

Antibody Phage Display Service

Phage display could be used to generate high-affinity monoclonal antibodies from all popular antibody production species, including but not limited to human, mouse, rat, rabbit, chicken, llama, camel, alpaca, cow, dog, sheep, monkey, and shark.

• No species or antibody format restriction (VHH, scFv, Fab…)
• Animal-free antibody discovery
• Large library capacity from 10⁷ to over 10¹² and high affinity from 10^-7 to 10^-9
• Fast process and ease of development
• Available for antigen presented on the surface of whole cells or tissues

How to Choose Suitable Monoclonal Antibody Service

Our customizable service options can handle all levels of project complexity. Whether you have a simple or challenging antigen, need a few or many mAb clones, or have specific screening requirements, rest assured that our antibody experts will do their best to tailor a custom mAb project to perfectly suit your research requirements.

Our talented Ph.D. technical support team is available 24 hours a day to address any questions you might have regarding developing a custom mAb project.

Antigens Designed and Produced In-House

We purify native antigens and produce both recombinant proteins, VLP-Displayed antigens, synthetic peptides and small molecule conjugates in-house. That means we have complete control over their design and QC.

Our Areas of Expertise:

Monoclonal Antibody Production

We provide monoclonal antibody production service depending on your needs:

• from small to large scale
• in vivo (ascites production) or in vitro (hybridoma cell culture, recombinant antibody production, stable cell line generation)
• aseptic/low endotoxin purification
• under our quality management systems that meet the requirements of the US FDA's Quality System Regulation (QSR) (21 CFR Part 820) and ISO 9001.

Choosing the best monoclonal antibody production service will mainly depend on several criteria such as:

• the amount of antibody needed
• your final application
• your timeline
• your budget

Contact our PhD account managers and benefit from their long-standing experience in monoclonal antibody production to determine the most relevant strategy for your project.

Additional Services