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Adjuvant

Description

Magic™ Mouse Adjuvant is a novel immunization adjuvant specifically designed for rapid production of high titers of antibodies in mice. The adjuvant contains immune-stimulatory CpG DNA-short oligodeoxynucleotide that contain unmethylated cytosine-guanine dinucleotides. It is by far the most efficient mouse adjuvant for all types of immunogens tested, in terms of its rapidness in raising immune responses and the antibody tiers it produces. Magic™ Mouse Adjuvant, Enhanced is the modified versions of magic mouse adjuvant, incorporating additional immunostimulating reagents, to broaden the immune response profile to more antigens. Magic™ Mouse Ascites Adjuvant is a novel adjuvant specifically designed for large amount of mouse ascites production.

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Features of Magic Mouse Adjuvant

  • Highly effective—rapid production of high titers of antibodies.
  • Easy to use—No emulsion step required.
  • Safe—non-toxic adjuvant with no adverse side effects to animals.
  • Immunogen saving—much less immunogen required than with conventional methods.
  • Time saving—strong immune responses can be reached within 35 days after immunization.
  • Extremely valuable-in producing antibodies against conformational epitopes.
Magic Mouse AdjuvantFreund's adjuvant
EmulsionNot requiredRequired
Immunization Methodintramuscularsubcutaneous or intraperitoneal
Immunization Timeline2-5 weeks8-10 weeks
Injection Times23-4
Immunogen Dosage1-20 ug per injection20-50 ug per injection
Toxicity NoYes

Contents & Storage

Magic™ Mouse Adjuvant is supplied as a ready-to-use solution and is shipped at ambient temperature. Upon arrival, it should be stored at 2-8°C.

Note: This product is supplied for research or manufacturing applications only and is not intended for clinical use.

References

  1. Edward A. Greenfield. Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 2013.
  2. Martin-Martin I, Chagas A C, Guimaraes-Costa A B, et al. Immunity to LuloHya and Lundep, the salivary spreading factors from Lutzomyia longipalpis, protects against Leishmania major infection[J]. PLoS pathogens, 2018, 14(5): e1007006.
  3. Canepa G E, Molina-Cruz A, Yenkoidiok-Douti L, et al. Antibody targeting of a specific region of Pfs47 blocks Plasmodium falciparum malaria transmission[J]. NPJ vaccines, 2018, 3.
  4. Palomo C, Mas V, Thom M, et al. Influence of Respiratory Syncytial Virus F Glycoprotein Conformation on Induction of Protective Immune Responses[J]. Journal of virology, 2016, 90(11): 5485-5498.
  5. Bernardo L, Denomme G A, Shah K, et al. RhD Specific Antibodies Are Not Detectable in HLA-DRB1 Mice Challenged with Human RhD Positive Erythrocytes[J]. Advances in hematology, 2014.
  6. Ferreira V P, Vale V F, Pangburn M K, et al. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis[J]. Scientific reports, 2016, 6.
  7. Olga A B, Tavares D, Setiady J, et al. Antibodies and Assays for Detection of Folate Receptor 1: U.S. Patent Application 14/473,828[P]. 2014-8-29.
  8. Mendes-Sousa, Antonio F., et al. "The sand Fly salivary Protein lufaxin inhibits the early steps of the alternative Pathway of complement by Direct Binding to the Proconvertase c3b-B." Frontiers in immunology 8 (2017): 1065.
  9. Asojo, Oluwatoyin A., et al. "Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis." PLoS neglected tropical diseases 11.3 (2017): e0005374.
  10. Vigneron, Aurélien, et al. "Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens." Proceedings of the National Academy of Sciences 117.5 (2020): 2613-2621.
  11. Diaz, Luis A., et al. "A Lutzomyia longipalpis salivary protein induces cross-reactive antibodies to pemphigus autoantigen desmoglein-1." Journal of Investigative Dermatology (2020).
  12. Molina-Cruz, Alvaro, et al. "Plasmodium falciparum evades immunity of anopheline mosquitoes by interacting with a Pfs47 midgut receptor." Proceedings of the National Academy of Sciences 117.5 (2020): 2597-2605.
  13. Gomes, Fabio M., et al. "Double peroxidase and histone acetyltransferase AgTip60 maintain innate immune memory in primed mosquitoes." Proceedings of the National Academy of Sciences 118.44 (2021): e2114242118.
  14. Williams, Adeline E., et al. "Aedes aegypti Piwi4 Structural Features Are Necessary for RNA Binding and Nuclear Localization." International Journal of Molecular Sciences 22.23 (2021): 12733.
  15. Burnett, William J., et al. "Prior Exposure to Coxsackievirus A21 Does Not Mitigate Oncolytic Therapeutic Efficacy." Cancers 13.17 (2021): 4462.
  16. Grill, Francisca J., et al. "Development of a quantitative antigen assay to detect coccidioidal chitinase-1 (CTS1) in human serum." Open forum infectious diseases. Vol. 8. No. 7. US: Oxford University Press, 2021.
  17. Valenzuela-Leon, Paola Carolina, et al. "Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein." Viruses 14.2 (2022): 221.
  18. Hsieh, Ching-Lin, et al. "Structure-based design of prefusion-stabilized human metapneumovirus fusion proteins." Nature communications 13.1 (2022): 1-11.
  19. Skinner, Benjamin, et al. "Monoclonal antibodies to Cache Valley virus for serological diagnosis." PLoS neglected tropical diseases 16.1 (2022): e0010156.
  20. Jugler, Collin, et al. "Potential for a Plant-Made SARS-CoV-2 Neutralizing Monoclonal Antibody as a Synergetic Cocktail Component." Vaccines 10.5 (2022): 772.
Using Magic Mouse Adjuvant
Cat. NoProduct NameUnit SizeDatasheetPrice
CDN-A001Magic Mouse Adjuvant1 mLPDF pdfInquiry
CDN-A001EMagic Mouse Adjuvant, Enhanced1 mLPDF pdfInquiry
CDN-CA1Magic Cellular Immunity Adjuvant1 mLPDF pdfInquiry
CDN-AS10 Magic Mouse Ascites Adjuvant 10 mLPDF pdfInquiry
CDN-AS500 Magic Mouse Ascites Adjuvant 500 mLPDF pdfInquiry

Other Adjuvant

Immunobiology Grade

  • For vaccination of sensitive animals
  • Adjuvant for laboratory animals
  • Adjuvant for antibody production

Vaccine Grade

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  • PRR Ligands-based adjuvant

DNA Vaccination

  • Genetic adjuvants
  • DNA vaccine plasmid

Material safety data sheets (MSDS)

MSDS