HEK 293 HCP ELISA Kit
Regulatory status: For research use only, not for use in diagnostic procedures.
COA
2. All other reagents should be stored at 2°C to 8°C for stability until the expiration date printed on the kit.
3. Reconstituted wash solution is stable until the expiration date of the kit.
4. After prolonged storage, you may notice a salt precipitate and/or yellowing of the wash concentrate. These changes will not impact assay performance. To dissolve the precipitate, mix the wash concentrate thoroughly and dilute as directed in the 'Preparation of Reagents' section.
The lower limit of quantitation (LLOQ) is defined as the lowest concentration, where concentration coefficients of variation (CVs) are less than 20%. The LLOQ is ~2.0 ng/mL.
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Q: Which specific epitopes does the kit target?
A: This assay kit detects protein residuals in HEK293 cells, preventing contamination from host proteins in biological products. Multiple proteins are targeted simultaneously. The antibodies have broad coverage of approximately 90% to HEK 293 HCPs, as determined.
Q: What is the precision of the kit?
A: Precision on duplicate samples should yield average % coefficients of variation of less than 10% for samples in the range of 10-200 ng/mL. CVs for samples less than 10 ng/mL may be greater than 10%.
Q: What is the typical shelf life?
A: 6 months
Q: Are there any known interferences with any buffers?
A: Your diluents should not have sodium azide as a preservative nor significant detergent concentrations as these will reduce assay accuracy.
Q: Are there any considerations in terms of salinity/pH etc. for the buffers we need to keep in mind when loading our samples?
A: CD offers assay specific diluents for each of its kits.The formulation of diluents should be a neutral pH with some carrier protein added to block non-specific adsorptive losses of the analyte. Use of just PBS or TBS without a carrier protein can be problematic because the analyte diluted in the range of the assay (ng/mL) can very significantly adsorb to the dilution tube resulting in low recovery!
Q: When I previously asked about the linear range of the standard curve, I was referring to what portion of the sigmoid is the linear region (e.g. within 25-50% of the top standard concentration?).
A: We don't typically evaluate true upper LLOQ. I would suggest not attempting to quantify sample higher than the top point of any of our kits. In this kit the top standard would be 200 ng/mL.
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