Anti-PI(3)P monoclonal antibody
Mouse Anti-PI(3)P monoclonal antibody for ELISA, IF
COA
Specifications
Antibody Isotype
IgG2a
Clone
42-23-0
Species Reactivity
N/A
Immunogen
PI(3)P conjugated to KLH
Conjugate
Unconjugated
Applications
Application Notes
ELISA, IF
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Images
DMABB-JX103 and an isotypic antibody was used and decorated the outer leaflet of fixed human-washed platelets plasma membrane as assessed by confocal microscopy.
Primary antibody: DMABB-JX103 at 1:100 dilution.
Secondary antibody: Goat anti mouse IgG polyclonal antibody at 1:300 dilution.
Primary antibody: DMABB-JX103 at 1:100 dilution.
Secondary antibody: Goat anti mouse IgG polyclonal antibody at 1:300 dilution.
Immunofluorescence analysis of PI(3)P puncta in HeLa cells in basal (DMEM) using DMABB-JX103 @ 1:300 dilution
cells were fixed in 2% w/v paraformaldehyde, permeabilized with 20 μM digitonin, and blocked with 5% v/ FBS.
Primary antibody: DMABB-JX103 @ 1:300 dilution
Secondary antibody: Goat anti mouse IgG polyclonal antibody [AF555]
cells were fixed in 2% w/v paraformaldehyde, permeabilized with 20 μM digitonin, and blocked with 5% v/ FBS.
Primary antibody: DMABB-JX103 @ 1:300 dilution
Secondary antibody: Goat anti mouse IgG polyclonal antibody [AF555]
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
Citations
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