Sample
Insect cell-baculovirus expression system
Intended Use
It' s applicable to biological products based on insect cell-baculovirus expression system, including but not limited to recombinant proteins, vaccines, gene therapy AAV vectors, etc.
Contents of Kit
No. | Components | Size | Storage Conditions |
1 | Sf9 HCP Standard | Lyophilized powder × 3 vials | 2-8°C |
2 | Coated Plate | 8 wells × 12 strips | 2-8°C |
3 | Standard Reconstitution Solution | 1.5 mL × 2 tubes | 2-8°C |
4 | Dilution Buffer | 50 mL × 1 vial | 2-8°C |
5 | Concentrated Buffer (10×) | 25 mL × 2 vials | 2-8°C |
6 | Sf9 HCP Enzyme-labeled Antibody (100×) | 120 μL × 1 tube | 2-8°C |
7 | TMB Substrate | 12 mL × 1 vial | 2-8°C |
8 | Stop Solution | 6 mL × 1 vial | 2-8°C |
9 | Sealing Film | 5 pieces | 2-8°C |
10 | Instructions for Use | 1 copy | |
Storage
Sealed kits are valid for 12 months at 2-8°C.
Storage requirements for unsealed components are as follows:
Microtiter plate: The unsealed microtiter plate strip shall be sealed and stored in a zip lock bag together with desiccant, and it can be stable for 30 days at 2-8°C after validation.
Reconstitution of calibrator: The calibrator can be stored at 2-8°C on the day of dissolution. After use, transfer to ≤ -18°C for storage on the day of use. Repeated freezing and thawing should not exceed 3 times.
Citations
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Shimojima, M; Morikawa, S; et al. Generation of monoclonal antibodies against a feline CD antigen (CD4) expressed by a recombinant baculovirus. JOURNAL OF VETERINARY MEDICAL SCIENCE 59:467-469(1997).
Li, YP; Ramirez, S; et al. Efficient Infectious Cell Culture Systems of the Hepatitis C Virus (HCV) Prototype Strains HCV-1 and H77. JOURNAL OF VIROLOGY 89:811-823(2015).