CDK5 Activity Assay Kit

Sample

Extracted samples

Species Reactivity

Human

Intended Use

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Contents of Kit

1. CDK5/p25: 2 μg, -80°C. Avoid multiple freeze/thaw cycles!
2. 5x Kinase assay buffer 1: 1.5 ml, -20°C. Avoid multiple freeze/thaw cycles!
3. ATP (500 μM): 100 μl, -20°C. Avoid multiple freeze/thaw cycles!
4. 10x CDK substrate peptide 1: 500 μl, -20°C. Avoid multiple freeze/thaw cycles!
5. 96-well plate, white: 1, Room Temp.

Storage

Up to 6 months when stored as recommended.

General Description

CDK5 is an unconventional member of the CDK family. It is expressed in post-mitotic neurons, and its activity is regulated by neuron-specific activators p35/p25 and p39 rather than the cyclins. It has been suggested that CDK5 plays several roles in the nervous system, including Tau aggregation and tangle formation. The CDK5 Assay Kit is designed to measure CDK5 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent. The CDK5 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK5/p25 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.

General Notes

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.