Anti-SNCA monoclonal antibody
Mouse anti-Human SNCA monoclonal antibody for WB, IHC-P, ELISA
Specifications
Clone
3C3B22
Species Reactivity
Human
Immunogen
Recombinant protein corresponding to human SNCA.
Conjugate
Unconjugated
Applications
Application Notes
ELISA (1:10000); WB (1:500-1:2000); Immunohistochemistry (1:200-1:1000) & The optimal working dilution should be determined by the end user.
Images
HC-P analysis of brain tissue by SNCA antibody.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SNCA antibody at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Neuropil is positively stained.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SNCA antibody at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Neuropil is positively stained.
Target
Alternative Names
SNCA; synuclein, alpha (non A4 component of amyloid precursor); PARK1, PARK4, Parkinson disease (autosomal dominant, Lewy body) 4; alpha-synuclein; alpha synuclein; NACP; PD1; synuclein alpha-140; non A-beta component of AD amyloid; PARK1; PARK4; MGC11098
Entrez Gene ID
UniProt ID
Product Background
Antigen Description
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimers disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene.
Pathway
Alpha-synuclein signaling, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyloids, organism-specific biosystem; Disease, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Parkinsons disease, organism-specific biosystem;
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
Citations
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| Product Name | Cat. No. | Applications | Host Species | Datasheet | Price | Add to Basket |
|---|---|---|---|---|---|---|
| Anti-Mouse IgG + IgM + IgA polyclonal antibody [HRP] | CPBT-68126GM | ELISA | Goat |
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| Anti-Mouse IgG + IgM + IgA polyclonal antibody [FITC] | CPBT-68125GM | FC | Goat |
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| Product Name | Cat. No. | Applications | Host Species | Datasheet | Price | Add to Basket |
|---|
Winton, MJ; Joyce, S; et al. Characterization of tau pathologies in gray and white matter of Guam parkinsonism-dementia complex. ACTA NEUROPATHOLOGICA 111:401-412(2006).
Sigurdsson, EM; et al. Tau Immunotherapy and Imaging. NEURODEGENERATIVE DISEASES 13:103-106(2014).
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Catalog # DCABH-13532
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