Species Reactivity
Human, Mouse, Rat
Intended Use
The Synuclein Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Synuclein protein expression profile in cells. The kit can be used for measuring the relative amounts of Synuclein in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Synuclein.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 Plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-Synuclein Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 Seals
Citations
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Anderson, LR; Betarbet, R; et al. PARK10 candidate RNF11 is expressed by vulnerable neurons and localizes to Lewy bodies in Parkinson disease brain. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY 66:955-964(2007).
An, YR; Tang, LL; et al. A Photoelectrochemical Immunosensor Based on Au-Doped TiO2 Nanotube Arrays for the Detection of alpha-Synuclein. CHEMISTRY-A EUROPEAN JOURNAL 16:14439-14446(2010).