AAV8 Titration ELISA Kit
Regulatory status: For research use only, not for use in diagnostic procedures.
COA
Sample
cell culture supernatants, purified virus preparations
Species Reactivity
N/A
Intended Use
Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of AAV serotype 8 particles in cell culture supernatants and purified virus preparations.
Contents of Kit
1.Microtiter Plate, 12 × 8-well-strips, coated with mouse monoclonal antibody to AAV8 in aluminum bag with desiccant, 1 plate. Ready- to-use.
2.AAV8 standard, lyophilized, 3 vials. Reconstitute before use.
3.Assay Buffer 20×, 50 ml. Dilute before use.
4.Anti-AAV8 Antibody, 12ml. Ready- to-use.
5.Anti-Human IgG Fc Conjugate, 12ml. Ready- to-use.
6.TMB Substrate, 6 ml × 2. Ready-to-use.
7.Stop Solution, 7 ml. Ready-to-use.
2.AAV8 standard, lyophilized, 3 vials. Reconstitute before use.
3.Assay Buffer 20×, 50 ml. Dilute before use.
4.Anti-AAV8 Antibody, 12ml. Ready- to-use.
5.Anti-Human IgG Fc Conjugate, 12ml. Ready- to-use.
6.TMB Substrate, 6 ml × 2. Ready-to-use.
7.Stop Solution, 7 ml. Ready-to-use.
Storage
Store the test kit and components at 2-8°C. The unopened reagents are stable at 2-8°C until the indicated expiry date.
Detection Range
0-2.65E+09vg/ml
General Description
Adeno-associated viruses (AAV) are non-pathogenic ssDNA viruses, which are subject of intense studies as viral vectors for gene therapy. The virus transduces a variety of dividing and non-dividing cells showing long-term gene expression with low cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinson's, Canavan disease) showing no serious vector-related adverse effects. Methods for the characterization of AAV preparations currently include titration ELISA, qPCR, ddPCR, DNA dot blot, determination of transducing units, infectious center assay, SDS-PAGE or electron microscopy.
Immunotitration by Creative Diagnostics' AAV8 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV8 wild-type virions, AAV8 recombinant virions or assembled and intact empty AAV8 capsids.
Immunotitration by Creative Diagnostics' AAV8 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV8 wild-type virions, AAV8 recombinant virions or assembled and intact empty AAV8 capsids.
Standard Curve
Citations
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Marsic, D; Zolotukhin, S; et al. High Accuracy Biodistribution Analysis of AAV Variants by Double Barcode Sequencing. MOLECULAR THERAPY 22:S26-S27(2014).
Tamai, H; Miyake, K; et al. A single injection of AAV-8 vector expressing IL-24 efficiently suppresses tumor growth mediated by specific mechanisms in MLL/AF4-positive ALL model mice. JOURNAL OF GENE MEDICINE 16:276-276(2014).
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