GTCDxᵀᴹ Anti-AAV5 Antibody ELISA Kit
Regulatory status: For research use only, not for use in diagnostic procedures.
COA
Sample
Serum
Species Reactivity
Human
Intended Use
The Anti-AAV5 ELISA is used as an analytical tool for qualitative determination of antibodies to AAV5 in serum.
Contents of Kit
| No. | Components | Size | Storage Conditions |
| 1 | AAV5 Capsid Coated Microtiter Plate | 12×8 wells | 2-8°C |
| 2 | Negative Control | lyophilized powder | 2-8°C |
| 3 | Anti-AAV5 Positive Control | lyophilized powder | 2-8°C |
| 4 | Cut-off Control | lyophilized powder | 2-8°C |
| 5 | Anti-human IgG Conjugate, ready to use | 12 mL | 2-8°C |
| 6 | Sample Diluent, ready to use | 50 mL | 2-8°C |
| 7 | Wash Buffer, 20 × concentrate | 50 mL | 2-8°C |
| 8 | TMB Substrate, ready to use | 2 × 6 mL | 2-8°C |
| 9 | Stop Solution, ready to use | 7 mL | 2-8°C |
| 10 | Instruction Manual | 1 |
Storage
1. All reagents should be stored at 2°C to 8°C for stability.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
Performance Characteristics
Function verification
Non-scaled Venn diagram depicting the relationship between total (tAb) and specific confirmed total antibodies in samples. The results are highly consistent, indicating that ELISA has high accuracy in detecting AAV antibodies.
Non-scaled Venn diagram depicting the relationship between total (tAb) and specific confirmed total antibodies in samples. The results are highly consistent, indicating that ELISA has high accuracy in detecting AAV antibodies.
Precision
General Description
Researchers have used AAV-based vectors in pre-clinical research and in clinical trials in which AAV-based vectors have demonstrated a good safety profile. AAVs have also demonstrated lasting therapeutic gene expression following a single treatment in preclinical and clinical studies.
One of the major challenges in AAV-based gene therapy is the presence of circulating anti-AAV neutralizing antibodies, which can pre-exist in patients and may prevent successful gene transfer. High levels of circulating anti-AAV neutralizing antibodies can develop after a single administration of gene therapy and can prevent successful gene transfer in patients.
One of the major challenges in AAV-based gene therapy is the presence of circulating anti-AAV neutralizing antibodies, which can pre-exist in patients and may prevent successful gene transfer. High levels of circulating anti-AAV neutralizing antibodies can develop after a single administration of gene therapy and can prevent successful gene transfer in patients.
Citations
Have you cited DEIASL343 in a publication? Let us know and earn a reward for your research.
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Darambazar, G; Nakata, M; et al. Paraventricular NUCB2/nesfatin-1 is directly targeted by leptin and mediates its anorexigenic effect. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 456:913-918(2015).
Earley, LF; Kawano, Y; et al. Identification and Characterization of Nuclear and Nucleolar Localization Signals in the Adeno-Associated Virus Serotype 2 Assembly-Activating Protein. JOURNAL OF VIROLOGY 89:3038-3048(2015).
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