Intended Use
The Mouse anti-AAV8 ELISA is used as an analytical tool for qualitative determination of antibodies to AAV8 in Mouse serum.
Contents of Kit
No. | Components | Size | Storage Conditions |
1 | AAV8 Capsid Coated Microtiter Plate | 12×8 wells | 2-8°C |
2 | Negative Control, ready to use | 1 × 1 ml | 2-8°C |
3 | Mouse Anti-AAV8 Positive Control, ready to use | 1 × 1 ml | 2-8°C |
4 | Anti-mouse IgG Conjugate, ready to use | 1 × 12 ml | 2-8°C |
5 | Sample Diluent, ready to use | 1 × 50 ml | 2-8°C |
6 | Wash Buffer, 20 × concentrate | 1 × 50 ml | 2-8°C |
7 | TMB Substrate, ready to use | 2 × 6 ml | 2-8°C |
8 | Stop Solution, ready to use | 1 × 7 ml | 2-8°C |
9 | Instruction Manual | 1 | |
Storage
1. All reagents should be stored at 2°C to 8°C for stability.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
Citations
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Ye, GJ; Scotti, MM; et al. Herpes Simplex Virus Clearance During Purification of a Recombinant Adeno-Associated Virus Serotype 1 Vector. HUMAN GENE THERAPY CLINICAL DEVELOPMENT 25:212-217(2014).
1. Issa S.S., et al. Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells. 2023;12:785.
2. Ling, Q., et al. AAV-based in vivo gene therapy for neurological disorders. Nat Rev Drug Discov 22. 2023, 789–806.