Mouse Anti-AAV8 ELISA Kit(Quantitative)
Regulatory status: For research use only, not for use in diagnostic procedures.
COA
Sample
Serum
Species Reactivity
Mouse
Intended Use
The Mouse Anti-AAV8 ELISA is used as an analytical tool for quantitative detection of antibodies to AAV8 in Mouse serum.
Storage
1. All reagents should be stored at 2°C to 8°C for stability.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
Precision
Intra-Assay: CV＜15%
Inter-Assay: CV＜15%
Inter-Assay: CV＜15%
Detection Range
For mouse anti-AAV8 IgG in serum, the method has been demonstrated to be highly (>0.99) linear from 0 to 250 ng/mL.
Sensitivity
The Detection Threshold for the assay is 1.72 ng/mL. The assay sensitivity for undiluted samples corresponds to 34.4 ng/mL, because the serum samples are instructed to be diluted at 20-fold (1:20) before starting the assay.
Standard Curve
Citations
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Belur, L; Nan, ZH; et al. High Level Expression of Human Iduronidase Throughout the Brain in a Murine Model of Mucopolysaccharidosis Type I After Non-Invasive AAV-Mediated Gene Delivery to the CNS. MOLECULAR THERAPY 22:S236-S236(2014).
Sen, D; et al. Improving clinical efficacy of adeno associated vectors by rational capsid bioengineering. JOURNAL OF BIOMEDICAL SCIENCE 21:-(2014).
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